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论著:中药复方对Lewis肺癌移植鼠肿瘤微环境中吲哚胺2,3 双加氧酶表达的影响
Influence of a Traditional Chinese Medicine Herbal Compound on the Expression of Indoleamine 2,3 Dioxygenase in the Tumor Microenvironment of Transplanted Lewis Lung Cancer Mice
王成刚 蒋霆辉 陈越 范济辉 张微微 沈旭波
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作者单位:复旦大学附属中山医院分部放射科
中文关键字:中药;吲哚胺2,3 双加氧酶;肿瘤免疫
英文关键字:Traditional Chinese medicine; Indoleamine 2,3 dioxygenase; Tumor immunity
中文摘要:目的:研究中药复方对Lewis肺癌移植鼠肿瘤微环境中吲哚胺2,3 二氧化酶(indoleamine 2,3 dioxygenase,IDO)表达的影响。方法:选用近交系C57BL/6纯系小鼠64只,将Lewis肺癌细胞悬液接种于小鼠右腋皮下建立肺癌模型,接种后小鼠随机分为4组:对照组、化疗组、中药复方组和中药复方加化疗组,通过应用免疫组化法及免疫印迹(Western blot)方法等多种实验技术,分别检测Lewis肺癌移植鼠肿瘤及淋巴组织中IDO表达水平。结果:中药复方及化疗药物顺铂均可在不同程度上影响IDO表达水平。同时中药复方与化疗药物顺铂具有协同作用,两者在不同环节调控调节性T细胞的作用与单一治疗相比存在一定优势。结论:中药复方与化疗在消除肿瘤微环境的局部免疫耐受中具有协同作用。
英文摘要:Objective: To study the influence of a traditional Chinese medicine (TCM) herbal compound on the expression of indoleamine 2,3 dioxygenase(IDO) in the tumor microenvironment of transplanted Lewis lung cancer mice. Methods:A total of 64 inbred C57BL/6 mice were chosen, which were inoculated Lewis lung carcinoma cell suspension in their right axilla subcutaneously. After that, they were divided randomly into 4 groups: control group, chemotherapy group, traditional Chinese medicine group and traditional Chinese medicine plus chemotherapy group. A variety of experimental techniques, such as immunohistochemistry and Westerm blot method were used to detect IDO expression level in the tumor and lymphoid tissues of transplanted Lewis lung cancer mice. Results: The traditional Chinese medicine and chemotherapy drug cisplatin could affect the IDO level in varying degrees. Traditional Chinese medicine had a synergistic effect with cisplatin, and there were therapeutic advantages in regulating regulatory T cells. Conclusions: The traditional Chinese medicine and chemotherapy have a synergistic effect in eliminating local immune tolerance in tumor microenvironment.
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