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论著:异氟醚麻醉对老年大鼠海马微管相关蛋白tau剪切的影响
Effect of Isoflurane on Tau Truncation in Hippocampus of Aged Rats
张晓光 薛张纲 仓静
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作者单位:复旦大学附属中山医院麻醉科
中文关键字:异氟醚;tau 蛋白;tau 剪切;半胱氨酸天冬氨酸特异性蛋白酶 3
英文关键字:Isoflurane; Tau proteins; Tau truncation; Caspase 3
中文摘要:目的:研究异氟醚麻醉对老年大鼠海马微管相关蛋白tau的剪切产物—Tau C3含量的影响及其可能机制。方法:将16只Sprague Dawley(SD)老年大鼠(>18个月)随机分为麻醉组(n=8)和对照组(n=8)。麻醉组大鼠接受浓度为1.5%~2%的异氟醚麻醉,时间为6 h;对照组除吸入气体中不含异氟醚外,其余处理与麻醉组相同。采用免疫组化多重标记法以及免疫印迹法检测Tau C3和激活的半胱氨酸天冬氨酸特异性蛋白酶 3 ( caspase 3)的表达,并观察两者在神经元内是否共同表达。结果:麻醉组的Tau C3 阳性细胞数[(5.6±2.4)个/高倍视野]显著高于对照组[(2.5±1.2)个/高倍视野],P<0.05。麻醉组Tau C3免疫印迹条带的相对光密度显著高于对照组。免疫组化多重标记的结果显示95%的Tau C3阳性细胞内无激活的caspase 3。麻醉组和对照组老年大鼠海马内激活的 caspase 3的含量无显著差异。结论:异氟醚麻醉可导致老年大鼠海马tau蛋白剪切产物Tau C3的增加,但Tau C3的增加与caspase 3激活无关。
英文摘要:Objective: To investigate the effect of isoflurane on tau truncation in hippocampus of aged rats and the possible mechanism. Methods:Sixteen aged Sprague Dawley(SD) rats (age >18 months) were randomly allocated to either anesthesia group (n=8) or control group (n=8). The rats in the anesthesia group underwent isoflurane anesthesia (1.5%-2%) for 6 h. The rats in the control group inhaled vehicle gas for 6 h. Immunofluorescent multiple labeling was employed to study the immunoreactivity of Tau C3, cleaved caspase 3, as well as the colocalization of them. Immunoblotting was used to semi quantify Tau C3 and cleaved caspase 3. Results: The density of Tau C3 positive cells were (5.6±2.4)/high power fields(HPF) in the anesthesia group and (2.5±1.2)/HPF in the control group. The relative optical density of Tau C3 in the anesthesia group was significantly higher than that in the control group. Immunofluorescent multiple labeling did not show colocalization of Tau C3 and cleaved caspase 3 in 95% of Tau C3 positive cells. The relative optical density of cleaved caspase 3 in both groups was low and the inter group difference was not statistically significant. Conclusions: Isoflurane anesthesia can enhance the tau truncation at D421 in the hippocampus of aged rats, and the process is not correlated with caspase 3.
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