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论著:肺炎衣原体诱发肺癌模型的建立
Study on Lung Cancer Model Induced by Chlamydia Pneumoniae
储德节 潘春峰 郭水根 王静 余竹元
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作者单位:复旦大学附属金山医院呼吸内科
中文关键字:肺炎衣原体;苯并芘;肺癌;动物模型
英文关键字:Chlamydia pneumoniae; Benzopyrene; Lung cancer; Animal model
中文摘要:目的:建立肺炎衣原体(Chlamydia pneumoniae, Cpn)诱发大鼠肺癌模型。方法:采用Cpn菌株TW 183感染的方法以及TW 183感染联合苯并芘(Benzopyrene,Bp)的方法建立肺癌Wistar大鼠模型。采用微量免疫荧光法检测大鼠血清中Cpn特异性抗体,采用聚合酶链式反应的方法检测大鼠肺癌组织中的Cpn DNA,采用酶联免疫吸附法检测大鼠肺癌组织中的Cpn特异性抗原。结果:单独Cpn感染大鼠的Cpn感染率为72.9%(35/48),Cpn感染联合Bp大鼠的Cpn感染率为76.7%(33/43);两组的肺癌发生率分别为14.6%(7/48)和44.2%(19/43),均显著高于对照小鼠的肺癌发生率(P=0.012,P<0.001)。结论:本研究成功建立Cpn感染诱发大鼠肺癌模型。
英文摘要:Objective: To build a rat model of lung cancer induced by Chlamydia pneumoniae(Cpn) infection. Methods:Cpn(TW 183) was injected into the lungs of the rats with or without the combination of Benzopyrene(Bp). Cpn antibodies(Cpn IgA, IgG and IgM) in rat serum were detected by the method of microimmunofluorescence(MIF). The Cpn DNA and Cpn Ag in lung cancer tissues were detected by polymerase chain reaction(PCR)and enzyme linked immunosorbent assay(ELISA), respectively. Results: The incidences of Cpn infection were 72.9%(35/48) in rats injected with Cpn and 76.7%(33/43) in rats injected with Cpn and Bp; and the incidences of lung cancer in them were 14.6%(7/48) and 44.2%(19/43), respectively, all of which were significantly higher than the controls(P=0.012, P<0.001, respectively). Conclusions: A rat model of lung cancer induced by Chlamydia pneumoniae infection has been successfully built.
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