网络编辑平台

编辑出版

《中国临床医学》编辑部

  地址:上海市枫林路179号18号楼501室

  邮编:200032

  电话:021-60267570

  邮箱:(1)zglcyx@126.com; (2)zglcyxfb@126.com

作者园地

在线调查

您喜欢的投稿方式调查


相关下载

您所在位置:首页->过刊浏览->第19卷第1期


论著:大鼠小体积肝移植模型的建立及技术改进
Model of 30% Small for Size Liver Transplantation in Rats and Its Technical Modification
宋康 高强 周俭 贺轶锋 孙琦蛮 杨国欢 史颖弘 肖永胜 谭长军 丁振斌 吴志全 樊嘉
点击:1272次 下载:0次
作者单位:复旦大学附属中山医院肝外科
中文关键字:肝移植;小体积供肝;大鼠;动物模型
英文关键字:Liver transplantation;Small for size graft liver;Rat;Animal model
中文摘要:目的:建立稳定的大鼠30%比例小体积肝移植模型。方法:采用SD大鼠,“二袖套法”行30%比例原位肝移植25例。经腹主动脉插管原位快速灌注获取供肝,冷缺血时间设定为1 h。结果:术后2 d存活率和1周存活率分别为72%(18/25)和64%(16/25)。结论:成功建立了大鼠30%比例的小体积肝移植模型,该模型手术成功率较高、便于操作,与临床部分肝移植(如活体肝移植)的手术十分相似,是研究肝移植缺血再灌注损伤的理想途径。
英文摘要:Objective:To establish the model of 30% small for size liver transplantation in rats. Methods: A total of 25 pairs of Sprague Dawley (SD) rats were performed 30% orthotopic liver transplantation (OLT) using Kamada’s 2 cuff method. The graft liver was procured by situ rapid perfusion through abdominal aorta. The time for cold ischemia was set as 1 hour. Results: After transplantation, 72%(18/25) of the recipients survived for 2 days or above and 64%(16/25) of the recipients were still alive in 1 week. Conclusions: We have successfully established the model of 30% small for size liver transplantation in rats with improvement in the methods. This model is stable, workable and replicable with high probability of success. The operation has similar procedures compared with clinical partial liver transplantation, such as living donor liver transplantation; and it is an ideal way for research on improving ischemia reperfusion injury (IRI) in liver transplantation and promoting liver regeneration.
读者评论

      读者ID: 密码:   
我要评论:
主办单位:复旦大学附属中山医院
联系地址:上海市枫林路179号18号楼501室 邮编:200032
联系电话:021-60267570 E-Mail:(1)zglcyx@126.com; (2)zglcyxfb@126.com
版权所有©2009-2010 《中国临床医学》杂志编辑部 沪ICP备1103249号
本系统由北京菲斯特诺科技有限公司设计开发
您是本站第1269991名访问者