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论著:季德胜蛇药对小鼠树突状细胞功能的影响
Regulatory Effects of Ji De Sheng She Yao on the Function of Dendritic Cell of Mice
谭晓慧 李阳 汪晓莺 周跃 沈轶瑶 汤伟
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作者单位:南通大学附属医院传染科
中文关键字:季德胜蛇药;树突状细胞;小鼠;环磷酰胺
英文关键字:Ji De Sheng She Yao; Dendritic cell; Mouse; Cyclophosphamide
中文摘要:目的:观察季德胜蛇药对小鼠树突状细胞(DCs)功能的调节作用。方法:48只BALB-c小鼠随机分为4组,每组各12只,分别为正常对照组、环磷酰胺模型组、治疗1组、治疗2组。各组均以灌胃给药,2次/d,连续21 d,正常对照组和造模组给予同体积的0.9%氯化钠液,治疗组分别给予相应剂量的蛇药治疗。给药第15天起,除正常对照组外各组小鼠均予以环磷酰胺30 mg/kg皮下注射,连续7 d制备免疫功能低下模型。比较4组间DCs表型、DCs功能和细胞因子白介素12(IL-12)的浓度。结果:与模型组比较,治疗组小鼠DCs的表面分子主要组织相容性抗原复合物(MHC)-Ⅱ、CD11c的表达水平显著升高、刺激T淋巴细胞增殖的能力以及分泌IL-12均显著增强。结论:季德胜蛇药可提高免疫抑制小鼠树突状细胞的免疫功能。
英文摘要:Objective: To study the regulatory effects of Ji De Sheng SheYao(JSD), a Chinese patent medicine, on the function of dendritic cell of mice.Methods: Forty-eight BALB-c mice were randomly divided into four groups (12 mice in each group): the normal control group, model group, treatment group 1 and treatment group 2. The drugs were given by intragastric gavage in every group twice every day, lasting twenty-one days. The normal control group and the model group were given the same volume of normal saline, the mice of the two treatment groups were respectively treated with drug(JSD) in corresponding dosages. From the 15th day, all experimental mice expect those of the normal control group were administered with the cyclophosphamide (30 mg/kg,iH)for seven days. The surface molecules of dendritic cells (DCs) the function of DCs and the concentration of interleukin-12(IL-12) were measured.Results: The expression of major histocompatibility complex(MHC)-Ⅱ, CD11c in the treatment group was significantly higher than that in model group. The abilities of DCs to stimulate T lymphocyte proliferation of mice in the treatment group were markedly higher than that of mice in model group; The contents of IL-12 in DCs’s supernatants of treatment group were significantly higher than those in model group.Conclusions: JSD can increase the DCs’ immune function of immune-suppressed mice.
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