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论著:血管紧张素Ⅱ-1型受体基因多态性与宁夏汉族原发性高血压病的相关性研究
Study for Association between the Angiogenesis Ⅱ Type I Receptor Gene Polymorphism and Essential Hypertension in Hans of Ningxia
陈丽娜 徐清斌 马萍
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作者单位:宁夏医科大学
中文关键字:原发性高血压;血管紧张素Ⅱ-1型受体;基因多态性
英文关键字:Essential hypertension; Angiogenesis Ⅱtype 1 receptor; Gene polymorphism
中文摘要:目的:研究血管紧张素Ⅱ-1型受体(angiogenesis Ⅱ type 1,AT1R)基因A1166C多态性与宁夏自治区汉族原发性高血压病(essential hypertension,EH)的关系。方法:采用多聚酶链式反应(polymerase chain reaction,PCR)结合限制性片段长度多态性(restriction fragment length poly orphism,RFLP)方法检测160例原发性高血压病患者( EH 组)和176 例血压值正常者(对照组) 的外周血白细胞DNA AT1R(A1166C)的基因多态性,统计分析该位点不同基因型及等位基因频率在EH组和对照组中的分布。结果:AA、AC 和CC 基因型分布频率在EH组中分别为93.1%、6.3%和0.6%;在对照组中分别为87.5%、11.4%和1.1%,2组比较差异无统计学意义(P>0.05);A1166 与1166C 等位基因频率在EH 组中分别为96.3%和3.8%;在对照组中分别为93.2%和6.8% 2组比较差异无统计学意义(P>0.05)。结论:AT1R 基因A1166C多态位点分子变异与宁夏自治区汉族易感原发性高血压病无显著相关性。
英文摘要:Objective: To investigate the relationship between the polymorphism of angiontensin Ⅱ type 1 receptor(AT1R)gene A1166C and essential hypertension(EH)in Hans in Ningxia Province, China. Methods: The genomic DNAs were extracted from the peripheral blood of 336 peoples in Ningxia Region. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods were used to detect the A1166C polymorphism of AT1R gene in Hans including 160 patients with EH and 176 normal controls. The frequencies of genotype distribution in EH and normal groups were studied. Results: The distribution genotype frequencies of AA, AC and CC were 93.1%, 6.3% and 0.6% in hypertension groups, respectively, and those in normal groups were 87.5%, 11.4% and 1.1%,respectively, which showed no significant difference(P>0.05); The allelic frequencies of A and C were 96.3% and 3.8%,respectively in hypertension group,while 93.2% and 6.8% in the normal group. The allele frequencies of A/ C were no significant differences to above data in two groups (P>0.05). Conclusions: It is no significant association found between the A1166C polymorphism of AT1R gene and essential hypertension in Hans of Ningxia.
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