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论著:缺血和缺氧诱导离体SD乳鼠心肌细胞凋亡发生的实验研究
Study of Apoptosis in the Myocyte of Neonat Rat Induced by Ischemia in Vitro
陶玙婧 江蓓湖 丘洪 赵军 郑世营 徐德民 王春生
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作者单位:上海中医药大学附属普陀医院老年科
中文关键字:缺血 心肌细胞 凋亡
英文关键字:Ischemia Myocyte Apoptosis
中文摘要:目的:通过建立一种新的离体SD乳鼠心肌细胞的缺氧模型,探讨心肌细胞缺氧、缺血损伤后的心肌细胞凋亡及其时序分布特点。方法:SD乳鼠的培养心肌细胞随机分为5组:对照组,缺氧12 h组(I 12h)、缺氧24 h组(I 24h),缺氧48 h组(I 48h)以及缺氧72 h组(I 72h)。应用 TUNEL 法染色和透射电镜检测心肌细胞凋亡的改变。结果:I 12 h组心肌细胞中见少量的TUNEL阳性细胞,随缺氧时间的延长,TUNEL阳性细胞数量逐渐增多。I 12h组的心肌细胞凋亡指数(AI)与对照组相比差异有统计学意义(P<0.01), I 48h组、I 72h组心肌细胞的AI值分别可达(24.4±2.41)%和(45.4±4.22)%。透射电镜观察各分析时相点心肌细胞,凋亡心肌细胞在I 24h组出现,I 48h组、I 72h组最多见。结论:乳鼠心肌细胞缺氧模型可导致心肌细胞凋亡,凋亡程度和心肌细胞缺氧时间有关。细胞凋亡在心肌缺血、缺氧心肌细胞死亡中起重要作用。
英文摘要:Objective: To develop a new kind of model of ischemic neonate rat myocytes in order to compare the timing and extent of apoptosis ischemic rat myocytes in vitro.Methods:The neonatal rat myocytes were randomly divided into 5 groups: control groups, ischaemic 12h group (I 12h), ischaemic 24h group (I 24h), ischaemic 48h group (I 48h) and ischaemic 72h group (I 72h). The cultured myocytes of each group were taken to detect apoptosis of myocytes by the TUNEL method and transmission electron microscopy (TEM). Results: As early as 12h after hypoxia insult in this model, TUNELpositive myocytes were detected. The cultured neonatal rat myocytes apoptosis indexes (AI) of the I 12h group was significantly higher than that of the control group (P<0.01), Furthermore, the AI of the I 48h group and the I 72h group was (24.4±2.14)% and (45.4±4.22)%, respectively. TEM examination showed that apoptosis occurred in some myocytes of the I 12h group and reached a peak in the myocytes of the I 48h group and the I 72h group.Conclusion:These results indicate that ischaemia can induce apoptosis of cultured neonatal rat myocytes and the efficiency of apoptosis is related to the timecourse of cultured myocytes after ischaemic insult. Apoptosis is an important form of the death of the cultured neonatal rat myocytes after ischaemic insult.
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